Directions for setting up of the Mushroom Biosphere ®™

• PARTS LIST: 1 main body with fittings, 1 screw top cap, 1 ultrasonic fogger /ionizer, 1 HEPA air pump, 2 air line hoses, 2 ½” hoses, (2 incubator tubs, 2 grommets, 2 water heaters, if ordered) and large grow chamber (55cubic liters) pre-drilled and thread tapped with HEPA filter. Plus the accessory bag which contains 2 liter bag of magic coconut coir growth booster ®™ , 2 large perlite bags, 2 large pre-mix vermiculite bags with mineral food, 4 HEPA filters, 4 rubber tie downs, 2 pair latex gloves, 1 spray bottle, 1 Lab mask, 1 alcohol towel, 1 bottle rubbing alcohol, 1 liquid crystal thermometer,1 lure lock syringe w/needle & Para film to seal syringe and 500ML of Deep Blue Water ®™

• ADDITIONAL ITEMS NEEDED (NOT INCLUDED) : 7 day/14 setting digital timer and a shop light. Pair of thick (leather) gloves or towel. (if you ordered the incubator tubs), vaseline and a pair of scissors.

• Connect all hoses to its proper fitting on the Biosphere, to the air pump and to the Grow Chambers.

• Screw the 2 straight (one end is threaded and the other side is slip) fittings into the bottom 2 holes on the grow chamber. Take the square HEPA filter and spray them with alcohol on both sides then place them over the straight fittings, and rubber band them into place with the enclosed bands.

Screw the white elbow into the middle top hole. This is the connector for the Biosphere.

Now it's time to clean the unit. It's highly recommended to put on some fresh clean clothes, than wash your hands and arms. Put on a pair of latex gloves that come with your kit and spray your arms and hands with the rubbing alcohol from your kit. Spray and wipe down the location you wish to set up your kit up with the alcohol also. Keep in mind you should put the setup on a table or a shelf in a draft free area for easier access and to keep contaminants and mold from falling into or around your set up. The job of spores is to drop, this way they find food and can reproduce. If you keep it off the floor, you are keeping it away from the contaminated environment. Gravity will pull a lot of the contaminants below your set up if it is on something a few feet from the floor. Remember to keep in mind that the air that you breathe is full of contaminants. Do not eat, drink or smoke around your set up. Beer especially is FULL of yeast, a major contaminant and on your breath if you are drinking while working around your kit.

That having been said you are now going to spray the inside of your grow chambers and the lids with some rubbing alcohol.

Wipe them down with a clean cloth and place the lid on your chamber, always cleaning the inside of everything first, placing the lid on, than cleaning the outside. Set it aside and do the same thing for all the tubs that came with your kit. A clean environment is your friend and you can never be too clean. It's better to be overly clean than not clean enough. Once you have your grow chambers clean you are going to do the same thing with your Biosphere, spraying the inside and outside with the rubbing alcohol to get rid of contamination.

Than wiping it down with a clean and soaked paper towel, starting with the inside, put on the lid and then clean the outside. It's a good practice to spray the inside of the tubes that came with your kit also to kill anything that might be in there.

• Fill the main body with store purchased purified bottled water and 1oz. Deep Blue Water ®™to the fill line on the clear hose

DO NOT OVER FILL!!!

The unit WILL NOT work properly if you do. DO NOT use tap water as it contains too many minerals and contaminants

and it will destroy the ionizer disk inside the MesBiosphere.

• Screw the cap back onto the Biosphere just until it stops. It does not need to be tight.

• Plug the ionizer into the transformer that plugs into the wall.

• Incubator setup (if you purchased the #2 or kits #4 ONLY)

#2#4

• Take the 2 tubs with 1 hole drilled in the bottom middle on the side, locate the 2 rubber grommets and put them into the hole, if not already in.

• Remove the water heater from the box. Using the Vaseline, apply a thin coating to the glass tube of the water heater as well as to the grommet. With the heavy gloves on, carefully and slowly insert the glass tube of the water heater into the rubber grommet. Do it slowly and gently to avoid breakage!!! (You may also use a towel to wrap the water heater in…but we can not be held responsible for any person that may get cut by broken glass because you did not protect your hands!!!! or used to much pressure.) See photos below.

• Remove the thermometer from the packaging and remove the sticker from the backside. Place it on the outside of the incubator tub about 1” from the bottom.

• Put approximately 2” of water in the incubator. Check around the grommet to make sure you have no leaks. If you think you have one slowly and gently rotate the grommet and water heater just a little bit.

• Now when you place the grow tub into the incubator make sure that the water does not overflow. You don't want the water too high!! About 3" to 4" up the side of the grow tub is plenty.(Just below the Co2 exhaust fitting, that is the black fitting with HEPA filter on it)

•Next screw in the 2 black fittings through both walls of the incubators and grow chambers,double check to see if the letters match each other "A" hole grow chamber goes inside "A" hole incubator. Spray both sides of the HEPA filter with rubbing alcohol and place the HEPA filter on each Co2 exhaust black fitting.

• Now the fun begins!!! Fill it with your favorite grow medium or use the medium we include and your favorite mushroom spawn, plug in the water heater and the mushroom biosphere ®™ , then watch and enjoy. (See below for setup info)

• IMPORTANT NOTES: Check the heat in the incubator to make sure it stays about 82 to 85 degrees for incubation (spawn run for the first 10 to 14 days) and 65 to 75 degrees for fruiting(after the first 10 to 14 days) If the incubator gets too high or to low simply adjust your water heater slowly until you get it right.

• Also…check the water level in you Biosphere ®™ daily… refill ONLY every 3 days NO MORE!!!, use bottled water and fill it back up to the fill line. (ADD 1 oz Deep Blue Water ®™ that came with the kit. If you run low you can always get more from www.mushroomtroll.com )

•Set up without the Incubators (main growing chambers with or without incubator)

(if you purchased the #1 #2 #3 or #4 kits )

#1#2

#3#4

• Once you have the unit set up and ready to go

• Take the bags of vermiculite, perlite and the Magic Coconut Coir Growth Booster®™ that comes with your kit

• (Vermiculite will be the large brown bag, perlite is the white bag and the Magic Coconut Coir Growth Booster®™ is the small brown bag) You will add 1000 ml. of bottled water to each of the perlite bags and 1500 ml. of bottled water to eachof the vermiculite bags . Add 500 ml of store bought bottle water to each bag of Magic Coconut Coir Growth Booster®™ Microwave for 15 minute on high. Let cool.

READ!!!!!

Please take note that newer microwaves will take a lot less time. Check your bags every 3 to 5 minutes.

The microwaving process should kill all the possible contaminants.

Magic Coconut Coir Growth Booster®™

• Make sure the bags are sealed, open just enough for steam to escape. (You don't want them to explode in your microwave.) Carefully place each bag into your microwave (if you place it on a plate it makes removal from the microwave easier) and let it run for 10 to 15 minutes on high to sterilize them (depending on your type of microwave). REMOVE CAREFULLY FROM MICROWAVE BECAUSE IT WILL BE HOT!!!!!! DO NOT OVER HEAT SO YOU DO NOT MELT A HOLE IN THE BAG. CHECK ON IT EVERY 3 TO 5 MINUTES AND IF YOUR MICROWAVE DOES NOT SPIN, ROTATE YOUR BAG 180 DEGREES SO THAT YOU GET EVEN HEATING THROUGHOUT. If a bag should tear DO NOT try to salvage what spills out. Immediately apply masking tape or duct tape to the hole and discard what fell out. It is now contaminated !!! Accidents happen so don't stress over it. Just tape the bag and throw away the mess that spilled out. What is inside the bag is still sterile and usable.

• Allow to cool so they are barely warm to the touch.

• DO EVERYTHING VERY CAREFULLY FROM THIS POINT ON !!!Put on your mask, rubber gloves and spray your arms and hands with rubbing alcohol. Open the lid of one of the grow chambers about 12" by sliding it backwards,

minimizing the air flow into the chamber but having enough room to pour the bag in. DO NOT place the entire bag inside the grow chamber. Instead POUR the bag in the chamber.The perlite (white) bag goes in first, gently open the 1 bag and pour it into the chamber. Slowly pour perlite in each chamber.

Open one lid at a time and close each lid as you finish working on that chamber. Now smooth out the perlite and make it level, using a flat hand . It is very important to have each layer nice and smooth. The more smooth and even the layers, the more even the flush of mushrooms will be. Do not pat or compact any layer. Take your hands out and close the lid. Do the same thing in the other chamber (make sure that NO clothing or skin touch ANY part of the inside of the tub). Remember to keep everything in your favor by being as cautious as possible and minimizing contamination as much as you can.

• Next lay your spawn down . You need at least 3-4 lbs. of fully colonized spawn per grow chamber.(if you don't have a spawn bag go to www.mushroomtroll.com and order Colonized Organic Rye Grain HEPA Filter Bags ( 8 Pounds of fresh live mycelium spawn. Spray the outside of the bag and a pair of scissors with rubbing alcohol. Also spray your arms and hands again. (Always spray your hands before they go back into the tubs.)• Break the spawn inside the bag without rupturing the bag (this a little tricky but fairly easy)

before

and after

• Cut open the top of the spawn bag(Colonized Organic Rye Grain HEPA Filter Bags ( 8 Pounds of fresh live mycelium spawn) and GENTLY put ½ of theColonized Organic Rye Grain HEPA Filter Bags ( 8 Pounds of fresh live mycelium spawnbag into 1 chamber on top of the perlite you laid before. Break the spawn into small pieces as you level it out in the chamber covering the whole thing.

Do the same in each chamber. Make sure you close the lid on the one you are not working in.

• Next lay your Magic Coconut Coir Growth Booster®™ pouring equal amounts and smoothing it out nice and even.

• Now slowly pour equal amounts of vermiculite (Brown) into the tubs. Gently pouring 1 bag in each tub and leveling it over the entire tub.

• It's now done. Close the lid and make sure you hear the snap from the lid (twice,one for each end)Let the Mushroom Biosphere ®™ unit run for 48 hours continuously and refill to the line as needed for the first 48 hours. After that you will need to plug the

Mushroom Biosphere ®™ into a timer that is set to run for 20 minutes every 2 hours for 2 chambers and 30 minutes every 2 hour for 4 chambers(not included in the kit). The pump needs to be plugged directly into the wall and allow to run for 24 hours a day.

With in a day or two of colonization you should take a black garbage bag, fold it to about 4” wide and tape it around the bottom of the incubator tub, then put it someplace that it gets plenty of light so that the ‘shrooms grow upwards, not back into the substrate (mushroom are phototropic,attracted to light and not photosynthesis ,use light as energy source)(using a 4 feet double bulb fixture from your local hardware store with 2 day light bulbs will produce more then enough light for 2 to 4 large grow chambers).Leave the light on for about 6 to 8 hours at a time. I prefer to use shop light with an electronic ballast, it is lighter and produces less heat than the one with an electric ballast. If you have problems telling the difference in the box. One is heavy and one is light. You want the light one.

• Remember to re-fill your Mushroom Biosphere ®™ every 3 days, NO more!!!! (ADD 1/2 oz Deep Blue Water ®™ that came with the kit every time you refill! If you run low you can always get more from www.mushroomtroll.com ). Don't worry if the Biosphere runs dry; the ionizer will not burn up. If it has no water it simply will not run. If you fill it too much you run the risk of having liquid accumulate in the bottom of your grow chamber which will cause water log and rot everything away.

•WATER LOG: If for any reason this would happen to you DON'T PANIC!!!

ALL YOU HAVE TO DO IS PUNCTURE A HOLE AT ONE END CORNER OF THE CHAMBER OF ABOUT 1/8 OF AN INCH IN DIAMETER AND LET THE EXTRA WATER DRIP IN TO A CONTAINER AND THROW THE WATER AWAY (in the garden is always my first choice.You never know what mother nature has in reserve for you ;)

• HAPPY SHROOMING!!!!!!

• DR. YOSTER

All the photos below are from a private collection of shrooms that where grown in the MesBiosphere®™(except the little one lol)